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PMID: 8197166 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ntk: a Csk-related protein-tyrosine kinase expressed in brain and T lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 11 ·1994-05-24 ·Pages 4975-9

Chow LM, Jarvis C, Hu Q, Nye SH, Gervais FG, Veillette A, Matis LA

Abstract

The activity of Src-related protein-tyrosine kinases is repressed by the phosphorylation of a conserved carboxyl-terminal tyrosine by another cytoplasmic protein-tyrosine kinase termed p50csk. In this study, we characterize Ntk, a protein-tyrosine kinase bearing striking similarities to p50csk. Like p50csk, Ntk possesses Src homology 3 and Src homology 2 domains and lacks the consensus tyrosine phosphorylation and myristoylation sites found in members of the Src family. Expression of ntk transcripts was maximal in brain, and was observed at significant levels in thymus and spleen. ntk RNA levels were dramatically reduced upon mitogenic stimulation of normal T lymphocytes and were minimal in transformed T-cell populations. Firm evidence that Ntk is a Csk-related enzyme was provided by the observation that it phosphorylated a Src-related polypeptide on the inhibitory carboxyl-terminal tyrosine. These findings indicate that Ntk is a Csk-related enzyme that may play an inhibitory role in the control of T-cell proliferation.

MeSH Terms
Amino Acid Sequence Animals Brain/metabolism Cells, Cultured Cloning, Molecular Lymphocyte Activation Mice Mice, Inbred BALB C Molecular Sequence Data Phosphorylation Protein-Tyrosine Kinases/biosynthesis Proto-Oncogene Proteins pp60(c-src) Sequence Homology, Amino Acid T-Lymphocytes/metabolism
Chemicals
Matk protein, mouse Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Chow L M
McGill Cancer Centre, McGill University, Montreal, PQ, Canada.
Jarvis C
Hu Q
Nye S H
Gervais F G
Veillette A
Matis L A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.11.4975
Published
1994-05-24
Pages
4975-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43912
Subset
IM
Databases
GENBANK
L27738
External Links
PubMed source DOI: 10.1073/pnas.91.11.4975 PMC Full Text View journal details
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