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PMID: 8198597 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Oxidation of low molecular weight chloroalkanes by cytochrome P450CAM.

Biochemical and biophysical research communications ·Vol. 201 ·No. 1 ·1994-05-30 ·Pages 373-8

Lefever MR, Wackett LP

Abstract

Cytochrome P450CAM from Pseudomonas putida G786 oxidized chlorinated ethanes and 1,2-dichloropropane. The rate of NADH oxidation decreased with decreasing chlorine substitution. The single detectable oxidation products of 1,1,1-trichloroethane and 1,2-dichloropropane were 1,1,1-trichloroethanol and chloroacetone, respectively. Organic product formation was largely uncoupled from NADH oxidation.

MeSH Terms

Camphor 5-Monooxygenase Cytochrome P-450 Enzyme System/metabolism Hydrocarbons, Chlorinated/metabolism Hydrogen Peroxide/metabolism Mixed Function Oxygenases/metabolism NAD/metabolism Oxidation-Reduction Pseudomonas putida/enzymology Substrate Specificity

Chemicals

Hydrocarbons, Chlorinated NAD Cytochrome P-450 Enzyme System Hydrogen Peroxide Mixed Function Oxygenases Camphor 5-Monooxygenase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lefever M R

Department of Biochemistry, Gortner Laboratory, University of Minnesota, St. Paul 55108.

Wackett L P

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1006/bbrc.1994.1711

Published

1994-05-30

Pages

373-8

Language

English

Region

United States

NLM ID

0372516

Subset

External Links

PubMed source DOI: 10.1006/bbrc.1994.1711 View journal details

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Oxidation of low molecular weight chloroalkanes by cytochrome P450CAM.

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