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PMID: 8202483 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A cell cycle-regulated inhibitor of cyclin-dependent kinases.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 12 ·1994-06-07 ·Pages 5291-5

Hengst L, Dulic V, Slingerland JM, Lees E, Reed SI

Abstract

Cyclin-dependent kinases (Cdks) previously have been shown to drive the major cell cycle transitions in eukaryotic organisms ranging from yeast to humans. We report here the identification of a 28-kDa protein, p28Ick (inhibitor of cyclin-dependent kinase), that binds to and inhibits the kinase activity of preformed Cdk/cyclin complexes from human cells. p28 inhibitory activity fluctuates during the cell cycle with maximal levels in G1 and accumulates in G1- and G0-arrested cells. These results suggest that control of the G1/S transition may be influenced by a family of Cdk inhibitors that include p28Ick and the recently described inhibitors p21Cip1/Waf1/Cap20 and p16Ink4.

MeSH Terms
CDC2-CDC28 Kinases Cell Cycle/drug effects Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinases Cyclins/metabolism HeLa Cells Humans Lovastatin/pharmacology Male Protein Kinase Inhibitors Protein Kinases/metabolism Protein Serine-Threonine Kinases
Chemicals
Cyclins Protein Kinase Inhibitors Lovastatin Protein Kinases Protein Serine-Threonine Kinases CDC2-CDC28 Kinases CDK2 protein, human Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hengst L
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
Dulic V
Slingerland J M
Lees E
Reed S I
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.12.5291
Published
1994-06-07
Pages
5291-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43980
Subset
IM
Grants
NIGMS NIH HHS · GM4006 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.12.5291 PMC Full Text View journal details
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