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PMID: 8209253 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Review

Forward and reverse genetic approaches to behavior in the mouse.

Science (New York, N.Y.) ·Vol. 264 ·No. 5166 ·1994-06-17 ·Pages 1724-33

Takahashi JS, Pinto LH, Vitaterna MH

Abstract

Modern molecular genetic and genomic approaches are revolutionizing the study of behavior in the mouse. "Reverse genetics" (from gene to phenotype) with targeted gene transfer provides a powerful tool to dissect behavior and has been used successfully to study the effects of null mutations in genes implicated in the regulation of long-term potentiation and spatial learning in mice. In addition, "forward genetics" (from phenotype to gene) with high-efficiency mutagenesis in the mouse can uncover unknown genes and has been used to isolate a behavioral mutant of the circadian system. With the recent availability of high-density genetic maps and physical mapping resources, positional cloning of virtually any mutation is now feasible in the mouse. Together, these approaches permit a molecular analysis of both known and previously unknown genes regulating behavior.

MeSH Terms
Animals Behavior, Animal Circadian Rhythm/genetics Female Genetic Techniques Genetics, Behavioral/methods Learning Long-Term Potentiation Male Mice Mice, Inbred Strains Mice, Knockout Mutagenesis
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Takahashi J S
Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208.
Pinto L H
Vitaterna M H
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.8209253
Published
1994-06-17
Pages
1724-33
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC3830945
Subset
IM
Grants
NIDCD NIH HHS · T32 DC000015 · United States
NIMH NIH HHS · MH49241 · United States
Howard Hughes Medical Institute · United States
NEI NIH HHS · EY08467 · United States
NIMH NIH HHS · MH39592 · United States
Corrections
CommentIn
PMID: 7809601
External Links
PubMed source DOI: 10.1126/science.8209253 PMC Full Text View journal details
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