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PMID: 8216240 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Malonyl-CoA metabolism in cardiac myocytes and its relevance to the control of fatty acid oxidation.

The Biochemical journal ·Vol. 295 ( Pt 1) ·1993-10-01 ·Pages 61-6

Awan MM, Saggerson ED

Abstract

1. Viable myocytes were obtained from rat hearts. Oxidation of [1-14C]palmitate by these cells could be decreased by the addition of glucose (5 mM) or lactate (2 mM). In the presence of glucose, insulin decreased and adrenaline increased palmitate oxidation. 2. The myocytes contained activities of ATP citrate-lyase, acetyl-CoA carboxylase and the condensing enzyme of the fatty acid elongation system. No fatty acid synthase activity was demonstrable in myocytes. 3. In rat hearts perfused with 5 mM glucose, malonyl-CoA content was acutely raised by insulin. In the presence of glucose+insulin, perfusion with palmitate or adrenaline decreased the malonyl-CoA content. 4. It is concluded that malonyl-CoA can be synthesized within cardiac myocytes and that the level of this metabolite can be acutely regulated. This is likely to have consequences for the regulation of carnitine palmitoyltransferase in the heart.

MeSH Terms
Acetyltransferases/metabolism Animals Cell Separation Cell Survival Cytosol/enzymology Fatty Acid Elongases Fatty Acids/metabolism Glucose/metabolism In Vitro Techniques Kinetics Lactates/metabolism Male Malonyl Coenzyme A/metabolism Myocardium/enzymology,metabolism Oxidation-Reduction Palmitates/metabolism Perfusion Rats Rats, Sprague-Dawley Subcellular Fractions/enzymology,metabolism Sympathomimetics/pharmacology
Chemicals
Fatty Acids Lactates Palmitates Sympathomimetics Malonyl Coenzyme A Acetyltransferases Fatty Acid Elongases Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Awan M M
Department of Biochemistry and Molecular Biology, University College London, U.K.
Saggerson E D
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2950061
Published
1993-10-01
Pages
61-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1134820
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2950061 PMC Full Text View journal details
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