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PMID: 8224829 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

P-lacW insertional mutagenesis on the second chromosome of Drosophila melanogaster: isolation of lethals with different overgrowth phenotypes.

Genetics ·Vol. 135 ·No. 1 ·1993-09-00 ·Pages 71-80

Török T, Tick G, Alvarado M, Kiss I

Abstract

A single P-element insertional mutagenesis experiment was carried out for the second chromosome of Drosophila melanogaster using the P-lacW transposon. Out of 15,475 insertions on the second chromosome, 2,308 lethal and 403 semilethal mutants (altogether 2,711) were recovered. After eliminating clusters, 72% of the mutants represent independent insertions. Some of the mutants with larval, prepupal or pupal lethal phases have a prolonged larval period and show gradual overgrowth of the imaginal discs, brain and/or the hematopoietic organs (lymph glands). In this paper, 16 overgrowth mutants are described. As revealed by in situ hybridization, none of the mutations corresponds to any of the previously known overgrowth mutations on the second chromosome.

MeSH Terms
Animals Chromosomes DNA Transposable Elements Drosophila melanogaster/genetics Female Genes, Lethal Genetic Complementation Test In Situ Hybridization Male Multigene Family Mutagenesis, Insertional Phenotype
Chemicals
DNA Transposable Elements
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Török T
Institute of Genetics, Hungarian Academy of Sciences, Szeged.
Tick G
Alvarado M
Kiss I
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/135.1.71
Published
1993-09-00
Pages
71-80
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205628
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/135.1.71 PMC Full Text View journal details
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