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PMID: 8225563 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Effect of prostaglandins and superoxide dismutase administration on oxygen free radical production in experimental acute pancreatitis.

Inflammation ·Vol. 17 ·No. 5 ·1993-10-00 ·Pages 563-71

Closa D, Bulbena O, Rosello-Catafau J, Fernandez-Cruz L, Gelpi E

Abstract

Oxygen free radicals and prostaglandins are implicated in the pathophysiology of acute pancreatitis, although their mechanisms of action remain unclear. We have studied the effect of administration of exogenous 16,16-dimethyl prostaglandin E2 and superoxide dismutase on oxygen free radical production in acute pancreatitis. For this purpose, five experimental rat groups were studied: group I, control; group II, sodium taurocholate-induced acute pancreatitis; group III, same as group II but with previous administration of 16,16-dimethyl prostaglandin E2; group IV, same as group II but with previous administration of indomethacin; and group V, same as group II but with previous administration of superoxide dismutase. In sodium taurocholate-treated rats, xanthine dehydrogenase is completely converted to xanthine oxidase within the first 5 min with subsequent oxygen free radical production while in 16,16-dimethyl prostaglandin E2-treated rats this enzyme transformation does not occur. In the superoxide dismutase-treated group xanthine oxidase activation is partially prevented. These data suggest that xanthine oxidase is the main source of oxygen free radicals, which contribute to extending the cellular damage in sodium taurocholate-induced acute pancreatitis.

MeSH Terms
16,16-Dimethylprostaglandin E2/pharmacology,therapeutic use Acute Disease Animals Edema/chemically induced,drug therapy Enzyme Activation Free Radicals Indomethacin/pharmacology,therapeutic use Lipase/blood Lipid Peroxidation/drug effects Male Pancreatitis/chemically induced,drug therapy,metabolism Rats Rats, Wistar Reactive Oxygen Species Superoxide Dismutase/pharmacology,therapeutic use Taurocholic Acid/toxicity Xanthine Dehydrogenase/metabolism Xanthine Oxidase/metabolism
Chemicals
Free Radicals Reactive Oxygen Species Taurocholic Acid Superoxide Dismutase Xanthine Dehydrogenase Xanthine Oxidase Lipase 16,16-Dimethylprostaglandin E2 Indomethacin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Closa D
Molecular Pathology Unit, CID-CSIC, Barcelona, Spain.
Bulbena O
Rosello-Catafau J
Fernandez-Cruz L
Gelpi E
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Article Info
Journal
Inflammation
Abbr.
Inflammation
ISSN
0360-3997
DOI
10.1007/BF00914194
Published
1993-10-00
Pages
563-71
Language
English
Region
United States
NLM ID
7600105
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00914194 View journal details
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