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PMID: 8225587 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular characterization and expression of p23 (OspC) from a North American strain of Borrelia burgdorferi.

Infection and immunity ·Vol. 61 ·No. 12 ·1993-12-00 ·Pages 5097-105

Padula SJ, Sampieri A, Dias F, Szczepanski A, Ryan RW

Abstract

We have found that sera from patients with early stages of Lyme disease contain predominant immunoglobulin M reactivity to a major 23-kDa protein (p23) from Borrelia burgdorferi 2591 isolated in Connecticut. To characterize this immunodominant antigen, we cloned and sequenced p23 and found it to be 83% identical by nucleotide sequence and 75% identical by amino acid sequenced to pC (recently renamed OspC), an abundantly expressed protein on the outer surface of PKo, a European strain of B. burgdorferi (B. Wilske, V. Preac-Mursic, S. Jauris, A. Hofmann, I. Pradel, E. Soutschek, E.Schwab, G. Will, and G. Wanner, Infect. Immun. 61:2182-2191, 1993). In addition, immunoelectron microscopy localized p23 to the outer membrane, confirming that p23 is the strain 2591 homolog of OspC. The North American strain B31, commonly used in serologic assays for Lyme disease, does not express OspC. Northern (RNA) blot analysis detected low levels of ospC mRNA in B31, and DNA sequencing of the ospC gene from B31 revealed a 54-bp deletion in the upstream regulatory region, possibly accounting for the low transcriptional activity of ospC. The ospC coding region from B31 was cloned and antibody-reactive OspC was expressed in Escherichia coli. An immunoglobulin M enzyme-linked immunosorbent assay using recombinant OspC as the target antigen shows promise for the serodiagnosis of early stages of Lyme disease.

Related Genes
MeSH Terms
Amino Acid Sequence Antibodies, Bacterial/biosynthesis Antibodies, Monoclonal Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins/genetics,immunology Base Sequence Borrelia burgdorferi Group/genetics,immunology,isolation & purification Cloning, Molecular DNA, Bacterial/genetics Enzyme-Linked Immunosorbent Assay/methods Gene Expression Genes, Bacterial Humans Immunoglobulin M/biosynthesis Lyme Disease/diagnosis,immunology,microbiology Microscopy, Immunoelectron Molecular Sequence Data North America Recombinant Fusion Proteins/genetics,immunology Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Antibodies, Bacterial Antibodies, Monoclonal Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins DNA, Bacterial Immunoglobulin M OspC protein Recombinant Fusion Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Padula S J
Department of Medicine, University of Connecticut Health Center, Farmington 06030-1310.
Sampieri A
Dias F
Szczepanski A
Ryan R W
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
Published
1993-12-00
Pages
5097-105
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC281288
Subset
IM
Grants
NIAMS NIH HHS · 5R29-AR39361 · United States
Databases
GENBANK
U01892, U01894
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