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PMID: 8226808 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of Trk binding sites for SHC and phosphatidylinositol 3'-kinase and formation of a multimeric signaling complex.

The Journal of biological chemistry ·Vol. 268 ·No. 31 ·1993-11-05 ·Pages 22963-6

Obermeier A, Lammers R, Wiesmüller KH, Jung G, Schlessinger J, Ullrich A

Abstract

Phosphotyrosine-containing synthetic peptides were used to identify the binding sites for cellular polypeptides involved in nerve growth factor receptor/Trk-mediated signal transduction. In vitro association of SHC and the p85 subunit of phosphatidylinositol 3'-kinase with the Trk tyrosine kinase was prevented only by phosphorylated Y-490- and Y-751-containing peptides, respectively. In spite of the close proximity of the p85 binding site to that of phospholipase C gamma (Y-785), both target proteins are able to interact with the same receptor molecule simultaneously.

Related Genes

SHC trk

MeSH Terms

Amino Acid Sequence Base Sequence Binding Sites Binding, Competitive Cell Line Humans In Vitro Techniques Macromolecular Substances Molecular Sequence Data Mutagenesis, Site-Directed Oligodeoxyribonucleotides/chemistry Peptide Fragments/chemistry Phosphatidylinositol 3-Kinases Phosphoproteins/metabolism Phosphotransferases (Alcohol Group Acceptor)/metabolism Proteins/metabolism Proto-Oncogene Proteins/metabolism Receptor Protein-Tyrosine Kinases/metabolism Receptor, trkA Receptors, Nerve Growth Factor/metabolism Signal Transduction Structure-Activity Relationship

Chemicals

Macromolecular Substances Oligodeoxyribonucleotides Peptide Fragments Phosphoproteins Proteins Proto-Oncogene Proteins Receptors, Nerve Growth Factor Phosphatidylinositol 3-Kinases Phosphotransferases (Alcohol Group Acceptor) Receptor Protein-Tyrosine Kinases Receptor, trkA

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Obermeier A

Department of Molecular Biology, Max-Planck-Institut für Biochemie, Martinsried, Germany.

Lammers R

Wiesmüller K H

Jung G

Schlessinger J

Ullrich A

Article Info

Journal

The Journal of biological chemistry

Abbr.

J Biol Chem

ISSN

0021-9258

Published

1993-11-05

Pages

22963-6

Language

English

Region

United States

NLM ID

2985121R

Subset

External Links

PubMed source View journal details

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Identification of Trk binding sites for SHC and phosphatidylinositol 3'-kinase and formation of a multimeric signaling complex.

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