[An acellular vaccine from "Pseudomonas aeruginosa." I. Preparation and activity (author's transl)].

From a strain (72 V) of Pseudomonas aeruginosa, we have prepared an acellular vaccine P2, which showed a good efficiency against homologous experimental infection in mice. The extraction procedure is as follows: washed bacterial cells are suspended in 0,15 M NaCl and heated at 60 degrees centigrade for 1 hr; after centrifugation, the supernatant fluid is precipitated with one and five volumes of ethanol. This acellular vaccine possess the following properties: rapid efficiency (10 days) after a single or three inoculations of very small doses (ED50 = 0.014 mug per mouse); weak toxicity (LD50 = 1,148 mug per mouse, subcutaneous route); capacity for production of specific protective antibodies.