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PMID: 8230479 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The DNA-binding domain of simian virus 40 tumor antigen has multiple functions.

Journal of virology ·Vol. 67 ·No. 12 ·1993-12-00 ·Pages 7608-11

Wun-Kim K, Upson R, Young W, Melendy T, Stillman B, Simmons DT

Abstract

The DNA-binding domain of simian virus 40 tumor antigen has been previously shown to participate in a number of different activities. Besides being involved in binding to sequences at the viral replication origin, this domain appears to be required for nonspecific DNA binding, for structurally distorting origin DNA (melting and untwisting), and possibly for oligomerization of the protein into hexamers and double hexamers. We now provide evidence that it also takes part in unwinding origin DNA sequences, contributes a function specifically related to in vivo DNA replication, and perhaps supports the assembly of the virus or release of the virus from the cell. This 100-amino-acid domain appears to be an excellent model system for studying how a small region of a protein could have a number of distinct activities.

MeSH Terms
Antigens, Viral, Tumor/genetics,metabolism DNA Helicases/metabolism DNA Replication DNA, Viral/metabolism DNA-Binding Proteins/genetics,metabolism Mutation Simian virus 40/genetics,immunology
Chemicals
Antigens, Viral, Tumor DNA, Viral DNA-Binding Proteins DNA Helicases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wun-Kim K
School of Life and Health Sciences, University of Delaware, Newark 19716.
Upson R
Young W
Melendy T
Stillman B
Simmons D T
References (26)
26 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.12.7608-7611.1993
Published
1993-12-00
Pages
7608-11
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC238227
Subset
IM
Grants
NCI NIH HHS · CA13106 · United States
NCI NIH HHS · CA36118 · United States
External Links
PubMed source DOI: 10.1128/JVI.67.12.7608-7611.1993 PMC Full Text View journal details
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