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PMID: 8233821 Published · ppublish English Journal Article

Nucleosomal structure at hyperacetylated loci probed in nuclei by DNA-histone crosslinking.

Nucleic acids research ·Vol. 21 ·No. 20 ·1993-10-11 ·Pages 4734-8

Ebralidse KK, Hebbes TR, Clayton AL, Thorne AW, Crane-Robinson C

Abstract

Chemically induced histone-DNA crosslinking in nuclei is used to monitor structural changes in chromosomal domains containing hyperacetylated histones. Core particles harbouring the crosslinks are immunofractionated with antibodies specific for acetylated histones. Crosslinking is revealed by gel separation of tryptic peptides from core histones that carry 32P-labelled residual nucleotide. The large number of DNA-histone crosslinks retained indicates that acetylated core histone tails are not totally displaced from the DNA. Changes in the patterns of crosslinked peptides imply a restructuring of hyperacetylated histone-DNA interactions at several points within the nucleosome. This demonstrates that a distinct conformational state is adopted in acetylated nucleosomes, known to be concentrated at transcriptionally active loci.

MeSH Terms
Acetylation Animals Cell Nucleus/chemistry Chick Embryo DNA/chemistry,metabolism Histones/chemistry,metabolism Nucleosomes/chemistry Precipitin Tests
Chemicals
Histones Nucleosomes DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ebralidse K K
Biophysics Laboratories, University of Portsmouth, UK.
Hebbes T R
Clayton A L
Thorne A W
Crane-Robinson C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.20.4734
Published
1993-10-11
Pages
4734-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331498
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1093/nar/21.20.4734 PMC Full Text View journal details
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