Selection and some properties of recombinant clones of lambda bacteriophage containing genes of Drosophila melanogaster.

Nucleic acids research ·Vol. 3 ·No. 8 ·1976-08-00 ·Pages 2115-27

Ilyin YV, Tchurikov NA, Georgiev

Abstract

The lambdagt clones containing fragments of the Drosophila melanogaster genome were prepared and characterized by hybridization of their DNA with (I) lambdagt-cRNA; (2) lambdaC-cRNA; (3) Dm-cRNA; (4) the mRNA of D.melanogaster culture cells and (5) the stable cytoplasmic poly (A) RNA from the same source. The technique for a simple selection of hybrid clones is described. The hybridization with mRNA allows one to select the clones containing structural genes of D.melanogaster. It was found that in all cases when the clone contains the structural gene it also contains the reiterated base sequences of the D.melanogaster genome. Several clones containing D. melanogaster DNA fragments with a size of (2-4)x1O6 daltons hybridizing with a relatively large portion of mRNA were selected for further analysis.

MeSH Terms

Animals Clone Cells Coliphages/metabolism DNA/metabolism DNA, Recombinant DNA, Viral/metabolism Drosophila melanogaster/metabolism Genes Nucleic Acid Hybridization Poly A/metabolism RNA, Messenger/metabolism Recombination, Genetic Selection, Genetic Transformation, Genetic

Chemicals

DNA, Recombinant DNA, Viral RNA, Messenger Poly A DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ilyin Y V

Tchurikov N A

Georgiev

References (13)

13 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.8.2115

Published

1976-08-00

Pages

2115-27

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343066

Subset

External Links

PubMed source DOI: 10.1093/nar/3.8.2115 PMC Full Text View journal details

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Selection and some properties of recombinant clones of lambda bacteriophage containing genes of Drosophila melanogaster.

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