Localization of tetramethylphenylenediamine-oxidase in the outer cell wall layer of Neisseria meningitidis.

Journal of bacteriology ·Vol. 128 ·No. 1 ·1976-10-00 ·Pages 144-8

Devoe IW, Golchrist JE

Abstract

A highly active tetramethylphenylenediamine-oxidase has been found in association with the cell wall blebs, evaginations of the outer wall membrane, of Neisseria meningitidis. Isolated wall blebs consumed oxygen in the presence of ascorbate-tetramethylphenylenediamine but not in the presence of succinate, whereas cell envelope preparations are active in both substrates. The ratio of succinate dehydrogenase/tetramethylphenylenediamine-oxidase activities in preparations of envelopes was approximately 100 times that in isolated wall blebs, indicating that the outer membrane preparations were highly purified.

MeSH Terms

Cell Fractionation Cell Wall/enzymology Cyanides/pharmacology Neisseria meningitidis/enzymology,metabolism,ultrastructure Oxidoreductases/isolation & purification,metabolism Oxygen Consumption Succinate Dehydrogenase/metabolism Tetramethylphenylenediamine

Chemicals

Cyanides Oxidoreductases Succinate Dehydrogenase Tetramethylphenylenediamine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Devoe I W

Golchrist J E

References (10)

10 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.128.1.144-148.1976

Published

1976-10-00

Pages

144-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC232836

Subset

External Links

PubMed source DOI: 10.1128/jb.128.1.144-148.1976 PMC Full Text View journal details

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Localization of tetramethylphenylenediamine-oxidase in the outer cell wall layer of Neisseria meningitidis.

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