Home LiteratureArticle Details
PMID: 8248254 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Selective repression of transcriptional activators at a distance by the Drosophila Krüppel protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 23 ·1993-12-01 ·Pages 11361-5

Licht JD, Ro M, English MA, Grossel M, Hansen U

Abstract

The Krüppel (Kr) protein, bound at kilobase distances from the start site of transcription, represses transcription by RNA polymerase II in mammalian cells. Repression is monotonically dependent on the dose of Kr protein and the presence of Kr binding site(s) on the DNA. These data suggest an inhibitory protein-protein interaction between the Kr protein and proximal transcription factors. Repression by Kr depends on the specific activator protein driving transcription. In particular, Kr protein selectively represses transcription mediated by the Sp1 glutamine-rich activation domain, tethered to the promoter by a GAL4 DNA-binding domain, but does not repress transcription stimulated by the acidic GAL4 activator. We believe this represents repression by a quenching interaction between DNA-bound Kr protein and the activation region of Sp1, rather than competition between Sp1 and Kr for a limiting transcriptional component. Selective, context-related repression affords an added layer of combinatorial control of gene expression by sequence-specific transcription factors.

Related Genes
Kr
MeSH Terms
Animals Cell Line Chlorocebus aethiops DNA-Binding Proteins/physiology Drosophila Proteins Drosophila melanogaster/genetics Gene Expression Regulation Glutamine/chemistry In Vitro Techniques Kruppel-Like Transcription Factors Promoter Regions, Genetic RNA, Messenger/genetics Repressor Proteins/physiology Structure-Activity Relationship Trans-Activators/antagonists & inhibitors,chemistry Transcription Factors/physiology Transcriptional Activation
Chemicals
DNA-Binding Proteins Drosophila Proteins Kr protein, Drosophila Kruppel-Like Transcription Factors RNA, Messenger Repressor Proteins Trans-Activators Transcription Factors Glutamine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Licht J D
Laboratory of Eukaryotic Transcription, Dana-Farber Cancer Institute, Boston, MA.
Ro M
English M A
Grossel M
Hansen U
References (44)
44 references, click to expand
  1. Promoter specificity of basal transcription factors.
    Cell. 1992 Mar 20;68(6):1135-44 PMID: 1547507
  2. Differential regulation of transcription preinitiation complex assembly by activator and repressor homeo domain proteins.
    Genes Dev. 1992 Nov;6(11):2177-89 PMID: 1358759
  3. The products of the Drosophila gap genes hunchback and Krüppel bind to the hunchback promoters.
    Nature. 1989 Sep 28;341(6240):335-7 PMID: 2797150
  4. Distinct classes of transcriptional activating domains function by different mechanisms.
    Cell. 1990 Sep 21;62(6):1177-87 PMID: 2205398
  5. Early and late periodic patterns of even skipped expression are controlled by distinct regulatory elements that respond to different spatial cues.
    Cell. 1989 May 5;57(3):413-22 PMID: 2720776
  6. Transcriptional repression in eukaryotes.
    Trends Genet. 1990 Jun;6(6):192-7 PMID: 2196723
  7. Analysis of maternal effect mutant combinations elucidates regulation and function of the overlap of hunchback and Krüppel gene expression in the Drosophila blastoderm embryo.
    Development. 1989 Nov;107(3):651-62 PMID: 2612383
  8. A novel mediator between activator proteins and the RNA polymerase II transcription apparatus.
    Cell. 1990 Jun 29;61(7):1209-15 PMID: 2163759
  9. Molecular cloning and functional analysis of Drosophila TAF110 reveal properties expected of coactivators.
    Cell. 1993 Jan 29;72(2):247-60 PMID: 7678780
  10. Activation of class II gene transcription by regulatory factors is potentiated by a novel activity.
    Cell. 1991 Sep 6;66(5):981-93 PMID: 1889091
  11. Sequence-specific DNA-binding activities of the gap proteins encoded by hunchback and Krüppel in Drosophila.
    Nature. 1989 Sep 28;341(6240):331-5 PMID: 2507923
  12. Correlative changes in homoeotic and segmentation gene expression in Krüppel mutant embryos of Drosophila.
    EMBO J. 1986 Jul;5(7):1659-65 PMID: 16453692
  13. The structure and assembly of active chromatin.
    Trends Genet. 1990 Feb;6(2):52-6 PMID: 2186529
  14. The zygotic control of Drosophila pair-rule gene expression. II. Spatial repression by gap and pair-rule gene products.
    Development. 1989 Nov;107(3):673-83 PMID: 2612385
  15. Genetic isolation of ADA2: a potential transcriptional adaptor required for function of certain acidic activation domains.
    Cell. 1992 Jul 24;70(2):251-65 PMID: 1638630
  16. Gradients of Krüppel and knirps gene products direct pair-rule gene stripe patterning in the posterior region of the Drosophila embryo.
    Cell. 1990 Apr 20;61(2):309-17 PMID: 2331752
  17. T antigen repression of SV40 early transcription from two promoters.
    Cell. 1981 Dec;27(3 Pt 2):603-13 PMID: 6101224
  18. Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5706-10 PMID: 6255460
  19. Activators and targets.
    Nature. 1990 Jul 26;346(6282):329-31 PMID: 2142753
  20. Three hormone receptor-like Drosophila genes encode an identical DNA-binding finger.
    EMBO J. 1989 Oct;8(10):3087-94 PMID: 2555153
  21. Negative regulation by glucocorticoids through interference with a cAMP responsive enhancer.
    Science. 1988 Jul 15;241(4863):350-3 PMID: 2838908
  22. Transcriptional repression by the Drosophila even-skipped protein: definition of a minimal repression domain.
    Genes Dev. 1993 Mar;7(3):491-503 PMID: 8095483
  23. Complementary patterns of even-skipped and fushi tarazu expression involve their differential regulation by a common set of segmentation genes in Drosophila.
    Genes Dev. 1987 Nov;1(9):981-95 PMID: 2892761
  24. How eukaryotic transcriptional activators work.
    Nature. 1988 Oct 20;335(6192):683-9 PMID: 3050531
  25. Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1.
    Cell. 1991 May 17;65(4):551-68 PMID: 1851669
  26. The protein Id: a negative regulator of helix-loop-helix DNA binding proteins.
    Cell. 1990 Apr 6;61(1):49-59 PMID: 2156629
  27. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  28. Transcriptional regulation of a pair-rule stripe in Drosophila.
    Genes Dev. 1991 May;5(5):827-39 PMID: 2026328
  29. A mechanism for synergistic activation of a mammalian gene by GAL4 derivatives.
    Nature. 1990 May 24;345(6273):361-4 PMID: 2160609
  30. Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif.
    Cell. 1988 Dec 2;55(5):887-98 PMID: 3142690
  31. Gene regulation by proteins acting nearby and at a distance.
    Nature. 1986 Aug 21-27;322(6081):697-701 PMID: 3018583
  32. A novel spatial transcription pattern associated with the segmentation gene, giant, of Drosophila.
    EMBO J. 1989 May;8(5):1539-48 PMID: 2504582
  33. Chromatin as an essential part of the transcriptional mechanism.
    Nature. 1992 Jan 16;355(6357):219-24 PMID: 1731219
  34. A mediator required for activation of RNA polymerase II transcription in vitro.
    Nature. 1991 Apr 4;350(6317):436-8 PMID: 2011193
  35. Mechanism of transcriptional activation by Sp1: evidence for coactivators.
    Cell. 1990 Jun 29;61(7):1187-97 PMID: 2194667
  36. Krüppel requirement for knirps enhancement reflects overlapping gap gene activities in the Drosophila embryo.
    Nature. 1989 Sep 28;341(6240):337-40 PMID: 2797151
  37. Yeast alpha 2 repressor positions nucleosomes in TRP1/ARS1 chromatin.
    Mol Cell Biol. 1990 May;10(5):2247-60 PMID: 2183026
  38. Evidence for interaction of different eukaryotic transcriptional activators with distinct cellular targets.
    Nature. 1990 Jul 12;346(6280):147-52 PMID: 2142255
  39. Transcriptional control signals of a eukaryotic protein-coding gene.
    Science. 1982 Jul 23;217(4557):316-24 PMID: 6283634
  40. The -117 mutation in Greek HPFH affects the binding of three nuclear factors to the CCAAT region of the gamma-globin gene.
    EMBO J. 1988 Oct;7(10):3099-107 PMID: 3181130
  41. Regulation of a segmentation stripe by overlapping activators and repressors in the Drosophila embryo.
    Science. 1991 Nov 29;254(5036):1385-7 PMID: 1683715
  42. Selective inhibition of activated but not basal transcription by the acidic activation domain of VP16: evidence for transcriptional adaptors.
    Cell. 1990 Jun 29;61(7):1199-208 PMID: 2163758
  43. Activation and repression of transcription by the gap proteins hunchback and Krüppel in cultured Drosophila cells.
    Genes Dev. 1991 Feb;5(2):254-64 PMID: 1671661
  44. Drosophila Krüppel protein is a transcriptional repressor.
    Nature. 1990 Jul 5;346(6279):76-9 PMID: 2114551
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.23.11361
Published
1993-12-01
Pages
11361-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47982
Subset
IM
Grants
NCI NIH HHS · K11CA01272 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.23.11361 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2