Abstract
In single cells isolated from guinea-pig ileal smooth muscle, held under voltage clamp at -40 mV or -50 mV by patch pipette in the whole-cell recording mode, carbachol (CCh) evoked an oscillatory inward cationic current. The frequency of current oscillations increased with increasing CCh concentration. CCh-evoked current oscillations were followed very closely by oscillations in intracellular free Ca2+ estimated from the Indo-1 signal, and were abolished by inclusion of EGTA in the pipette solution. Ryanodine and heparin, but not nifedipine, blocked the generation of current oscillations. CCh-evoked current oscillations were abolished upon withdrawal of extracellular calcium and restored upon its reintroduction. Inclusion of GTP[gamma S] in the pipette solution caused the generation of an oscillatory inward current, which was blocked by ryanodine. The present results are consistent with the hypothesis that CCh-evoked cationic current is gated by activation of a G protein and is steeply dependent on [Ca2+]i, fluctuations in the release of Ca2+ from stores during carbachol's action produce oscillations in [Ca2+]i which cause similar oscillations in the cationic current.
MeSH Terms
Animals
Calcium/metabolism
Carbachol/pharmacology
Egtazic Acid/pharmacology
Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
Guinea Pigs
Heparin/pharmacology
Ileum/metabolism
Male
Membrane Potentials
Muscle, Smooth/metabolism
Nifedipine/pharmacology
Receptors, Cell Surface/metabolism
Ryanodine/pharmacology
Signal Transduction
Chemicals
Receptors, Cell Surface
Ryanodine
Guanosine 5'-O-(3-Thiotriphosphate)
Egtazic Acid
Carbachol
Heparin
Nifedipine
Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Komori S
Department of Veterinary Medicine, Gifu University, Japan.
Kawai M
Pacaud P
Ohashi H
Bolton T B
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