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PMID: 8255754 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A tRNA gene transcription initiation site is similar to mRNA and rRNA promoters in plant mitochondria.

Nucleic acids research ·Vol. 21 ·No. 22 ·1993-11-11 ·Pages 5012-9

Binder S, Brennicke A

Abstract

The gene for tRNA(Phe) is located 292 nucleotides upstream of the tRNA(Pro) gene in the Oenothera mitochondrial genome. Hybridization with in vitro capped primary transcripts indicates a transcription initiation site in the 5' region of the gene for tRNA(Phe). Primer extension experiments show the presence of precursor transcripts covering tRNA(Phe) and adjacent sequences up to a transcription initiation site 181 or 180 nucleotides upstream of the tRNA gene. The genomic sequence at this transcription initiation site contains the consensus motif derived for putative promoters of mitochondrial protein and rRNA coding genes in dicotyledonous plants. This sequence similarity suggests that tRNAs, rRNAs and mRNAs can be transcribed from homologous promoters in plant mitochondria.

MeSH Terms
Base Sequence DNA, Mitochondrial/genetics DNA-Directed RNA Polymerases/metabolism Molecular Sequence Data Plants/genetics Promoter Regions, Genetic RNA, Messenger/genetics RNA, Ribosomal/genetics RNA, Transfer, Phe/genetics RNA, Transfer, Pro/genetics Recombination, Genetic Restriction Mapping Transcription, Genetic
Chemicals
DNA, Mitochondrial RNA, Messenger RNA, Ribosomal RNA, Transfer, Phe RNA, Transfer, Pro DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Binder S
Institut für Genbiologische Forschung, Berlin, Germany.
Brennicke A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.22.5012
Published
1993-11-11
Pages
5012-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310611
Subset
IM
Databases
GENBANK
X74449, X74450
External Links
PubMed source DOI: 10.1093/nar/21.22.5012 PMC Full Text View journal details
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