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PMID: 82604 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mechanism of action of factor D of the alternative complement pathway.

The Journal of experimental medicine ·Vol. 148 ·No. 6 ·1978-12-01 ·Pages 1498-509

Lesavre PH, Müller-Eberhard HJ

Abstract

Factor D (C3 proactivator convertase) of human serum has been shown to be absolutely necessary for alternative pathway function, for activation of the C3/C5 convertase of that pathway and not to be a subunit of this enzyme. Factor D was found to be present in human plasma in active form only, at a concentration of 2 microgram/ml, and not to be controlled by plasma protease inhibitors or by spontaneous decay. Unlike trypsin, factor D cleaves and activates factor B only when it is in Mg++-dependent complex with C3b, has no esterolytic activity, and is unable to cleave the B chain of insulin. The alleged functional and antigenic relationship of factor D to alpha-thrombin could not be verified. The results of this study led to the description of the mechanism of action of factor D in terms of the cryptic site hypothesis.

MeSH Terms
Complement Activating Enzymes/metabolism Complement Activation Complement C3/metabolism Complement Factor D/analysis,immunology,metabolism Complement Pathway, Alternative Epitopes Humans Magnesium/metabolism Thrombin/immunology
Chemicals
Complement C3 Epitopes Complement Activating Enzymes CFD protein, human Complement Factor D Thrombin Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lesavre P H
Müller-Eberhard H J
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35 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.148.6.1498
Published
1978-12-01
Pages
1498-509
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2185104
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.148.6.1498 PMC Full Text View journal details
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