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PMID: 8262062 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A complex secondary structure in U1A pre-mRNA that binds two molecules of U1A protein is required for regulation of polyadenylation.

The EMBO journal ·Vol. 12 ·No. 13 ·1993-12-15 ·Pages 5191-200

van Gelder CW, Gunderson SI, Jansen EJ, Boelens WC, Polycarpou-Schwarz M, Mattaj IW, van Venrooij WJ

Abstract

The human U1A protein-U1A pre-mRNA complex and the relationship between its structure and function in inhibition of polyadenylation in vitro were investigated. Two molecules of U1A protein were shown to bind to a conserved region in the 3' untranslated region of U1A pre-mRNA. The secondary structure of this region was determined by a combination of theoretical prediction, phylogenetic sequence alignment, enzymatic structure probing and molecular genetics. The U1A binding sites form (part of) a complex secondary structure which is significantly different from the binding site of U1A protein on U1 snRNA. Studies with mutant pre-mRNAs showed that the integrity of much of this structure is required for both high affinity binding to U1A protein and specific inhibition of polyadenylation in vitro. In particular, binding of a single molecule of U1A protein to U1A pre-mRNA is not sufficient to produce efficient inhibition of polyadenylation.

MeSH Terms
Base Sequence Binding Sites Humans Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Precursors/metabolism Poly A/metabolism RNA Processing, Post-Transcriptional RNA, Messenger/metabolism RNA, Small Nuclear/metabolism RNA-Binding Proteins Ribonucleoprotein, U1 Small Nuclear/genetics,metabolism Sequence Alignment Sequence Homology, Nucleic Acid
Chemicals
Nucleic Acid Precursors RNA, Messenger RNA, Small Nuclear RNA-Binding Proteins Ribonucleoprotein, U1 Small Nuclear U1A protein Poly A
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
van Gelder C W
University of Nijmegen, Department of Biochemistry, The Netherlands.
Gunderson S I
Jansen E J
Boelens W C
Polycarpou-Schwarz M
Mattaj I W
van Venrooij W J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-12-15
Pages
5191-200
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413783
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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