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PMID: 8264545 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Determination of the size of the Azotobacter vinelandii chromosome.

Molecular & general genetics : MGG ·Vol. 241 ·No. 5-6 ·1993-12-00 ·Pages 719-22

Manna AC, Das HK

Abstract

The chromosome of Azotobacter vinelandii UW was digested separately with the rape cutter restriction endonucleases SwaI (5'-ATTTAAAT), PmeI (5'GTTTAAAC) and PacI (5'-TTAATTAA) and the products were separated by pulsed-field gel electrophoresis. The size of the chromosome was determined to be approximately 4.5 megabase pairs (Mb) based on the sum of the sizes of the restriction fragments. This is almost the same as the size of the chromosome of Escherichia coli. The inability of the undigested DNA to enter the gel has led us to infer that the chromosome is circular.

MeSH Terms
Azotobacter vinelandii/genetics Chromosomes, Bacterial/ultrastructure DNA, Bacterial/chemistry DNA, Circular/chemistry Electrophoresis, Gel, Pulsed-Field Restriction Mapping
Chemicals
DNA, Bacterial DNA, Circular
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Manna A C
Genetic Engineering Unit, Jawaharlal Nehru University, New Delhi, India.
Das H K
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13 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00279916
Published
1993-12-00
Pages
719-22
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00279916
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