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PMID: 8264607 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mismatch repair of heteroduplex DNA intermediates of extrachromosomal recombination in mammalian cells.

Molecular and cellular biology ·Vol. 14 ·No. 1 ·1994-01-00 ·Pages 400-6

Deng WP, Nickoloff JA

Abstract

Previous work indicated that extrachromosomal recombination in mammalian cells could be explained by the single-strand annealing (SSA) model. This model predicts that extrachromosomal recombination leads to nonconservative crossover products and that heteroduplex DNA (hDNA) is formed by annealing of complementary single strands. Mismatched bases in hDNA may subsequently be repaired to wild-type or mutant sequences, or they may remain unrepaired and segregate following DNA replication. We describe a system to examine the formation and mismatch repair of hDNA in recombination intermediates. Our results are consistent with extrachromosomal recombination occurring via SSA and producing crossover recombinant products. As predicted by the SSA model, hDNA was present in double-strand break-induced recombination intermediates. By placing either silent or frameshift mutations in the predicted hDNA region, we have shown that mismatches are efficiently repaired prior to DNA replication.

Related Genes
neo
MeSH Terms
Alleles Animals CHO Cells/metabolism Chromosome Mapping Cricetinae Crossing Over, Genetic/genetics DNA/genetics,metabolism DNA Repair/genetics Models, Genetic Mutation Neomycin/pharmacology Nucleic Acid Heteroduplexes/genetics,metabolism Phenotype Recombination, Genetic
Chemicals
Nucleic Acid Heteroduplexes DNA Neomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Deng W P
Department of Cancer Biology, Harvard University School of Public Health, Boston, Massachusetts 02115.
Nickoloff J A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.1.400-406.1994
Published
1994-01-00
Pages
400-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358389
Subset
IM
Grants
NCI NIH HHS · CA 54079 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/mcb.14.1.400-406.1994 PMC Full Text View journal details
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