Home LiteratureArticle Details
PMID: 8265357 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.

Nucleic acids research ·Vol. 21 ·No. 23 ·1993-11-25 ·Pages 5408-17

Blattner FR, Burland V, Plunkett G, Sofia HJ, Daniels DL

Abstract

We present the sequence of 176 kilobases of the Escherichia coli K-12 genome, from katG at 89.2 to an open reading frame (ORF) of unknown function at 92.8 minutes on the genetic map. This brings the total of contiguous sequence from the E. coli genome project to 500 kb (81.5 to 92.8 minutes). This segment contains 134 putative coding genes (ORFs) of which 66 genes were previously identified. Eight new genes--acs, pepE, and nrfB-G--were identified as well as the previously mapped gldA and alr genes. Still, 58 ORFs remain unidentified despite literature and similarity searches. The arrangement of proposed genes relative to possible promoters and terminators suggests 55 potential transcription units. Other features include 13 REP elements, one IRU (ERIC) repeat, 59 computer-predicted bends, 42 Chi sites and one new grey hole. Sixteen signal peptides were found, including those of lamB, btuB, and malE. Two ribosomal RNA loci, rrnB and rrnE, are located in this segment, so we have now sequenced four of the seven E. coli rRNA loci. Comparison of the rRNA loci reveals some differences in the ribosomal structural RNAs which are generally compatible with the proposed secondary structures.

Related Genes
argB argC argE argH birA btuB coaA gldA gltT katG murB murI nusG oxyR ppc rplA rplJ rplK rrfB rrlB rrsB secE trmA tufB udhA
MeSH Terms
Base Sequence DNA, Bacterial/genetics DNA, Ribosomal/genetics Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Nucleic Acid Conformation Open Reading Frames Protein Sorting Signals/chemistry Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid Restriction Mapping
Chemicals
DNA, Bacterial DNA, Ribosomal Protein Sorting Signals
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Blattner F R
Laboratory of Genetics, University of Wisconsin, Madison 53706.
Burland V
Plunkett G
Sofia H J
Daniels D L
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.23.5408
Published
1993-11-25
Pages
5408-17
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310579
Subset
IM
Grants
NHGRI NIH HHS · HG000301 · United States
Databases
GENBANK
L10328, L19201, M87049, U00006
External Links
PubMed source DOI: 10.1093/nar/21.23.5408 PMC Full Text View journal details
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