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PMID: 8276466 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of a new kinesin-related gene located at the centromeric end of the human MHC region.

Immunogenetics ·Vol. 39 ·No. 3 ·1994-00-00 ·Pages 194-200

Ando A, Kikuti YY, Kawata H, Okamoto N, Imai T, Eki T, Yokoyama K, Soeda E, Ikemura T, Abe K

Abstract

We previously reported the presence of a new gene (HSET) with an unknown function, in the centromeric side of the class II gene region of the human major histocompatibility complex (MHC). cDNA clones corresponding to the HSET gene were isolated from a human testis cDNA library. A 2.4 kilobase transcript from the HSET gene was abundantly expressed in testis, B-cell, T-cell, and ovary cell lines but was not detected in lung or stomach. Analysis of the nucleotide sequence of the HSET cDNA clones revealed significant similarity to kinesin-related proteins in yeast, Drosophila, and human. Its predicted amino acid sequence contains a domain with strong sequence similarity to the ATP-binding and motor domains of a plus end-directed microtubule motor protein, kinesin, which might be involved in mitotic chromosome segregation, suggesting that the HSET gene encodes a novel kinesin-related protein.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Line Centromere Chromosomes, Artificial, Yeast Chromosomes, Human, Pair 6 Cloning, Molecular DNA, Complementary/genetics Female Genes, MHC Class II/genetics Humans Kinesins/genetics Male Molecular Sequence Data Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid Tissue Distribution
Chemicals
DNA, Complementary Kinesins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Ando A
Department of Molecular Life Science, Tokai University School of Medicine, Kanagawa, Japan.
Kikuti Y Y
Kawata H
Okamoto N
Imai T
Eki T
Yokoyama K
Soeda E
Ikemura T
Abe K
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00241260
Published
1994-00-00
Pages
194-200
Language
English
Region
United States
NLM ID
0420404
Subset
IM
Databases
GENBANK
D14678
External Links
PubMed source DOI: 10.1007/BF00241260 View journal details
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