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PMID: 8280098 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phospholipase C in Dictyostelium discoideum. Identification of stimulatory and inhibitory surface receptors and G-proteins.

The Biochemical journal ·Vol. 297 ( Pt 1) ·1994-01-01 ·Pages 189-93

Bominaar AA, Van Haastert PJ

Abstract

A combined biochemical and genetic approach was used to show that phospholipase C in the cellular slime mould Dictyostelium is under dual regulation by the chemoattractant cyclic AMP (cAMP). This dual regulation involves stimulatory and inhibitory surface receptors and G-proteins. In wild-type cells both cAMP and guanosine 5'-[gamma-thio]triphosphate (GTP[S]) stimulated phospholipase C. In contrast, mutant fgd A, lacking the G-protein alpha-subunit G alpha 2, showed no stimulation by either cAMP or GTP[S], indicating that G alpha 2 is the stimulatory G-protein. In mutant fgd C cAMP did not stimulate phospholipase C, but stimulation by GTP[S] was normal, suggesting that the defect in this mutant is upstream of the stimulatory G alpha 2. Inhibition of phospholipase C was achieved in wild-type cells by the partial antagonist 3'-deoxy-3'-aminoadenosine 3',5'-phosphate (3'NH-cAMP). This inhibition was no longer observed in transformed cell lines lacking either the surface cAMP receptor cAR1 or the G-protein alpha-subunit G alpha 1; in these cells the agonist cAMP still activated phospholipase C. These results indicate that Dictyostelium phospholipase C is regulated via a stimulatory and an inhibitory pathway. The inhibitory pathway is composed of the surface receptor cAR1 and the G-protein G1. The stimulatory pathway consists of an unknown cAMP receptor (possibly the fgd C gene product) and the G-protein G2.

MeSH Terms
Animals Cyclic AMP/analogs & derivatives,pharmacology Dictyostelium/enzymology,genetics Enzyme Activation/drug effects GTP-Binding Proteins/physiology Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology Inositol 1,4,5-Trisphosphate/metabolism Inositol Polyphosphate 5-Phosphatases Mutation Phosphoric Monoester Hydrolases/metabolism Receptors, Cyclic AMP/genetics,physiology Type C Phospholipases/antagonists & inhibitors,metabolism
Chemicals
Receptors, Cyclic AMP Guanosine 5'-O-(3-Thiotriphosphate) 3'-deoxy-3'aminocyclic-3',5'-AMP Inositol 1,4,5-Trisphosphate Cyclic AMP Phosphoric Monoester Hydrolases Inositol Polyphosphate 5-Phosphatases Type C Phospholipases GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bominaar A A
Department of Biochemistry, University of Groningen, The Netherlands.
Van Haastert P J
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2970189
Published
1994-01-01
Pages
189-93
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1137809
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2970189 PMC Full Text View journal details
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