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PMID: 8282124 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The human sperm protein PH-20 has hyaluronidase activity.

FEBS letters ·Vol. 336 ·No. 3 ·1993-12-28 ·Pages 545-8

Gmachl M, Sagan S, Ketter S, Kreil G

Abstract

The PH-20 protein present on the membrane of guinea pig sperm was characterized using a monoclonal antibody [(1991) J. Cell Biol. 111, 2939-2949]. We have isolated the cDNA encoding the human PH-20 protein from a testis library. This cDNA was expressed in RK 13 cells using a vaccinia virus expression system. Cells expressing the human PH-20 protein possess hyaluronidase activity. Treatment with PI-PLC releases the hyaluronidase into the the medium with a concomitant large increase in enzymatic activity. These results demonstrate that the human PH-20 protein has hyaluronidase activity.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Adhesion Molecules/biosynthesis,isolation & purification,metabolism Cloning, Molecular DNA Primers Gene Library Guinea Pigs Humans Hyaluronoglucosaminidase/biosynthesis,metabolism Kinetics Male Molecular Sequence Data Phosphatidylinositol Diacylglycerol-Lyase Phosphoinositide Phospholipase C Phosphoric Diester Hydrolases/metabolism Polymerase Chain Reaction Recombinant Proteins/biosynthesis,isolation & purification,metabolism Spermatozoa/metabolism Testis/metabolism Vaccinia virus
Chemicals
Cell Adhesion Molecules DNA Primers Recombinant Proteins Phosphoric Diester Hydrolases Phosphoinositide Phospholipase C Hyaluronoglucosaminidase hyaluronidase PH-20 Phosphatidylinositol Diacylglycerol-Lyase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gmachl M
Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
Sagan S
Ketter S
Kreil G
Article Info
Journal
FEBS letters
Abbr.
FEBS Lett
ISSN
0014-5793
DOI
10.1016/0014-5793(93)80873-s
Published
1993-12-28
Pages
545-8
Language
English
Region
England
NLM ID
0155157
Subset
IM
External Links
PubMed source DOI: 10.1016/0014-5793(93)80873-s View journal details
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