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PMID: 8293977 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetic and phenotypic studies of hypomorphic lin-12 mutants in Caenorhabditis elegans.

Genetics ·Vol. 135 ·No. 3 ·1993-11-00 ·Pages 755-63

Sundaram M, Greenwald I

Abstract

The lin-12 gene of Caenorhabditis elegans is thought to encode a receptor for intercellular signals that specify certain cell fates during development. We describe several alleles of lin-12 that reduce but do not eliminate lin-12 activity (hypomorphic alleles). These alleles cause a novel egg-laying defective (Egl) phenotype in hermaphrodites as well as incompletely penetrant cell fate transformations seen with high penetrance in lin-12 null mutants. Characterization of the Egl phenotype revealed additional roles of lin-12 in the development of the egg-laying system that were not apparent from studying lin-12 null mutants: lin-12 activity is required for proper early vulval morphogenesis as well as for some unknown later aspect of egg-laying system development. Reversion of the Egl phenotype caused by one lin-12 hypomorphic allele was used to identify potential interacting genes as described in the accompanying paper.

Related Genes
lin-12
MeSH Terms
Alleles Animals Caenorhabditis elegans/genetics,growth & development,physiology Disorders of Sex Development/genetics Female Genes, Helminth Male Mutation Oviposition/genetics,physiology Phenotype Vulva/abnormalities,growth & development
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sundaram M
Department of Molecular Biology, Princeton University, New Jersey 08544.
Greenwald I
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24 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/135.3.755
Published
1993-11-00
Pages
755-63
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205718
Subset
IM
Grants
NIGMS NIH HHS · GM37602 · United States
External Links
PubMed source DOI: 10.1093/genetics/135.3.755 PMC Full Text View journal details
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