Rotary replication for freeze-etching.
Rotary replication has been adapted to freeze-etching and evaluated using T4 polyheads, erythrocyte ghosts, and chloroplast membranes. Conventional electron microscopy, electron diffraction, and optical diffraction and filtering indicate that platinum-carbon rotary replication renders radially symmetrical contrast and 25 A resolution to freeze-etched specimens so as to clarify subunit structure not normally evident in unidirectional shadow replicas.
7 references, click to expand
-
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.Arch Biochem Biophys. 1963 Jan;100:119-30 PMID: 14028302
-
Fracture faces in frozen outer segments from the guinea pig retina.Z Zellforsch Mikrosk Anat. 1968;91(4):586-603 PMID: 4882176
-
Recent progress in the freeze-etching technique.Philos Trans R Soc Lond B Biol Sci. 1971 May 27;261(837):121-31 PMID: 4399199
-
[Improved resolution in freeze-etching].Histochemie. 1969;20(2):133-42 PMID: 4901927
-
Maturation of the head of bacteriophage T4. IV. The proteins of the core of the tubular polyheads and in vitro cleavage of the head proteins.Virology. 1974 Dec;62(2):483-99 PMID: 4432376
-
Structure of the tubular variants of the head of bacteriophage T4 (polyheads). I. Arrangement of subunits in some classes of polyheads.J Mol Biol. 1972 Apr 14;65(3):469-88 PMID: 5023668
-
Application of the freeze-fracture technique to natural membranes.Methods Enzymol. 1974;32:35-44 PMID: 4614001
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
