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PMID: 8313893 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intrachromosomal homologous recombination in whole plants.

The EMBO journal ·Vol. 13 ·No. 2 ·1994-01-15 ·Pages 484-9

Swoboda P, Gal S, Hohn B, Puchta H

Abstract

A system to assay intrachromosomal homologous recombination during the complete life-cycle of a whole higher eukaryote was set up. Arabidopsis thaliana plants were transformed with a recombination substrate carrying a non-selectable and quantitatively detectable marker gene. The recombination substrates contain two overlapping, non-functional deletion mutants of a chimeric beta-glucuronidase (uidA) gene. Upon recombination, as proven by Southern blot analysis, a functional gene is restored and its product can be detected by histochemical staining. Therefore, cells in which recombination events occurred, and their progeny, can be precisely localized in the whole plant. Recombination was observed in all plant organs examined, from the seed stage until the flowering stage of somatic plant development. Meristematic recombination events revealed cell lineage patterns. Overall recombination frequencies typically were in the range 10(-6)-10(-7) events/genome. Recombination frequencies were found to differ in different organs of particular transgenic lines.

Related Genes
uidA
MeSH Terms
Arabidopsis/genetics Blotting, Southern Genes, Plant Genetic Markers Glucuronidase/genetics Organ Specificity/genetics Recombination, Genetic Transformation, Genetic
Chemicals
Genetic Markers Glucuronidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Swoboda P
Friedrich Miescher-Institut, Basel, Switzerland.
Gal S
Hohn B
Puchta H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-01-15
Pages
484-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394832
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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