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PMID: 8317546 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of integrin receptors in normal and neoplastic human brain.

The American journal of pathology ·Vol. 143 ·No. 1 ·1993-07-00 ·Pages 154-63

Paulus W, Baur I, Schuppan D, Roggendorf W

Abstract

We studied the immunohistochemical expression of integrin alpha and beta chains in the normal and neoplastic human brain. Normal astrocytes expressed alpha 2, alpha 3, alpha 6, beta 1, and beta 4 chains in some areas facing major interstitial tissues, but they were consistently negative for the other integrins examined (alpha 4, alpha 5, alpha V, alpha L, alpha M, alpha X, beta 2, beta 3). Neoplastic astrocytes in vivo and in vitro showed increased expression of alpha 3 and beta 1, and some also of alpha 5, alpha V, beta 3, and beta 4. Neoexpression of alpha 4 and reduced levels of beta 4 were detected in glioblastoma vascular proliferations compared with normal endothelial cells. Oligodendroglioma, ependymoma, choroid plexus papilloma, pituitary adenoma, and meningioma cells showed the same integrin pattern as their normal counterparts. Adhesion assays using the astrocytoma cell lines U-138 MG and U-373 MG revealed strong attachment to collagen types I to VI and undulin, which was inhibited by antibodies to beta 1, but not by those to alpha 2, alpha 3, alpha 6, and alpha V. We conclude that astrocytomas show increased levels or neoexpression of various integrins and strong attachment to various extracellular matrix components, which appears to be almost exclusively mediated by beta 1-integrins.

MeSH Terms
Antibodies/pharmacology Astrocytoma Brain Chemistry Brain Neoplasms/chemistry,pathology Cell Adhesion/immunology Cell Differentiation/drug effects Collagen/metabolism Connective Tissue/metabolism Glioma/pathology Glycoproteins/metabolism Humans Integrins/analysis,immunology,physiology Laminin/metabolism Tumor Cells, Cultured
Chemicals
Antibodies COL14A1 protein, human Glycoproteins Integrins Laminin Collagen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Paulus W
Institute of Pathology, University of Würzburg, Germany.
Baur I
Schuppan D
Roggendorf W
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1993-07-00
Pages
154-63
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1886949
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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