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PMID: 8319300 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and sequence of HSP30, a yeast heat-shock gene coding for a hydrophobic membrane protein.

Current genetics ·Vol. 23 ·No. 5-6 ·1993-00-00 ·Pages 435-42

Régnacq M, Boucherie H

Abstract

Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 amino-acid protein. No similarities to any previously known protein have been noted. The protein is very hydrophobic and is predicted to have a membraneous localisation. In agreement with this hypothesis, the analysis of membrane proteins from stationary-phase cells showed that a strain carrying this gene on a multicopy vector overproduces a protein of 30 kDa. This protein was recognized by antibodies directed against the N-terminal portion of the gene product. Considering its induction in response to heat shock and the apparent molecular weight of its product, this gene was designated HSP30.

Related Genes
HSP30
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Cloning, Molecular Codon DNA, Fungal/isolation & purification Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal Genes, Fungal HSP30 Heat-Shock Proteins Heat-Shock Proteins/genetics,metabolism Membrane Proteins/genetics,metabolism Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins
Chemicals
Codon DNA, Fungal Fungal Proteins HSP30 Heat-Shock Proteins HSP30 protein, S cerevisiae Heat-Shock Proteins Membrane Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Régnacq M
Laboratoire de Génétique, URA CNRS 542, Talence, France.
Boucherie H
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00312631
Published
1993-00-00
Pages
435-42
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Databases
GENBANK
M93123, X68488, X68489, X68490, X71675, X71676, X71677, X71678, X71679, X71680
External Links
PubMed source DOI: 10.1007/BF00312631 View journal details
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