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PMID: 8320241 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Characterization of the katG gene encoding a catalase-peroxidase required for the isoniazid susceptibility of Mycobacterium tuberculosis.

Journal of bacteriology ·Vol. 175 ·No. 13 ·1993-07-00 ·Pages 4255-9

Heym B, Zhang Y, Poulet S, Young D, Cole ST

Abstract

The isoniazid susceptibility of Mycobacterium tuberculosis is mediated by the product of the katG gene which encodes the heme-containing enzyme catalase-peroxidase. In this study, the chromosomal location of katG has been established and its nucleotide sequence has been determined so that the primary structure of catalase-peroxidase could be predicted. The M. tuberculosis enzyme is an 80,000-dalton protein containing several motifs characteristic of peroxidases and shows strong similarity to other bacterial catalase-peroxidases. Expression of the katG gene in M. tuberculosis, M. smegmatis, and Escherichia coli was demonstrated by Western blotting (immunoblotting). Homologous genes were detected in other mycobacteria, even those which are naturally insensitive to isoniazid.

Related Genes
katG
MeSH Terms
Amino Acid Sequence Bacterial Proteins Chromosome Mapping Drug Resistance, Microbial Genes, Bacterial/genetics Isoniazid/pharmacology Molecular Sequence Data Mycobacterium/enzymology,genetics Mycobacterium tuberculosis/drug effects,enzymology,genetics Peroxidases/genetics,immunology Sequence Analysis, DNA Sequence Homology, Amino Acid
Chemicals
Bacterial Proteins Peroxidases catalase HPI Isoniazid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Heym B
Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
Zhang Y
Poulet S
Young D
Cole S T
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.13.4255-4259.1993
Published
1993-07-00
Pages
4255-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204858
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.175.13.4255-4259.1993 PMC Full Text View journal details
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