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PMID: 8327465 Published · ppublish English Journal Article

Expression cloning in yeast of a cDNA encoding a broad specificity amino acid permease from Arabidopsis thaliana.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 13 ·1993-07-01 ·Pages 5944-8

Frommer WB, Hummel S, Riesmeier JW

Abstract

To study amino acid transport in plants at the molecular level, we have isolated an amino acid permease cDNA from Arabidopsis thaliana by complementation of a yeast mutant defective in proline uptake with a cDNA. The predicted polypeptide of 53 kDa is highly hydrophobic with 12 putative membrane-spanning regions and shows no significant homologies to other known transporters. Expression of the cDNA enables the yeast mutant to take up L-[14C]proline. Competition studies argue for a broad but stereospecific substrate recognition by the permease, which resembles neutral or general amino acid transport systems from Chlorella and higher plants. Both pH dependence and inhibition by protonophores are consistent with a proton symport mechanism.

MeSH Terms
Amino Acid Transport Systems Arabidopsis/enzymology,genetics,growth & development Base Sequence Biological Transport Cloning, Molecular Genes, Plant Genetic Complementation Test Membrane Transport Proteins/genetics,metabolism Molecular Sequence Data Proline/metabolism Sequence Analysis, DNA Substrate Specificity Yeasts/enzymology,genetics
Chemicals
Amino Acid Transport Systems Membrane Transport Proteins Proline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Frommer W B
Institut für Genbiologische Forschung, Abteilung Willmitzer, Berlin, Federal Republic of Germany.
Hummel S
Riesmeier J W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.13.5944
Published
1993-07-01
Pages
5944-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46843
Subset
IM
Databases
GENBANK
X67124
External Links
PubMed source DOI: 10.1073/pnas.90.13.5944 PMC Full Text View journal details
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