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PMID: 8332472 Published · ppublish English Comparative Study Journal Article

Disruption in the AU motif of the mouse TNF-alpha 3' UTR correlates with reduced TNF production by macrophages in vitro.

Nucleic acids research ·Vol. 21 ·No. 11 ·1993-06-11 ·Pages 2761-6

Jacob CO, Tashman NB

Abstract

Many cytokine mRNAs exhibit a conserved, AU-rich motif in the 3'-untranslated region (UTR) of the molecule. Such sequence elements have been implicated in the regulation of mRNA turnover and as potential translational regulators. We report on the identification of a 3 base pair insertion which disrupts the AU motif of the TNF-alpha gene in the NZW, B10.KPA44, SM/J and Mus spretus mice and an insertion of an 8 base pair sequence into the 3' AU motif of the IL-10 gene in the Mus Spretus mouse. The mutation in the AU motif of the TNF-alpha gene correlates with reduced production of this cytokine by peritoneal macrophages from these mouse strains.

MeSH Terms
Adenine Animals Base Composition Base Sequence Conserved Sequence DNA/genetics,isolation & purification Genome Liver/metabolism Macrophages/metabolism Mice/genetics Mice, Inbred Strains/genetics Molecular Sequence Data Muridae/genetics Polymerase Chain Reaction/methods Protein Biosynthesis RNA, Messenger/genetics,metabolism Regulatory Sequences, Nucleic Acid Spleen/metabolism Tumor Necrosis Factor-alpha/biosynthesis,genetics Uracil
Chemicals
RNA, Messenger Tumor Necrosis Factor-alpha Uracil DNA Adenine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jacob C O
Institute of Immunology and Biological Sciences, Syntex Research, Palo Alto, CA 94304.
Tashman N B
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.11.2761
Published
1993-06-11
Pages
2761-6
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309616
Subset
IM
Databases
GENBANK
M90457, M90458
External Links
PubMed source DOI: 10.1093/nar/21.11.2761 PMC Full Text View journal details
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