Menaquinone (vitamin K2) biosynthesis: cloning, nucleotide sequence, and expression of the menC gene from Escherichia coli.
The benzenoid aromatic compound o-succinylbenzoic acid is formed by dehydration of the prearomatic compound 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid by the enzyme o-succinylbenzoate synthase, encoded by the menC gene. A 1.3-kb PstI-PvuII fragment was found to complement the menC mutation. The complete nucleotide sequence of this fragment revealed a single open reading frame of 954 bp capable of encoding a 35-kDa protein. A consensus sequence for a ribosomal binding site but no promoter consensus sequences were found. However, the first base of the initiating codon of this open reading frame overlaps the upstream menB gene termination codon, suggesting an operon-like organization for these genes. Consistent with this suggestion, the menB promoter can initiate transcription of the menC gene.
14 references, click to expand
-
A new intermediate in naphthoquinone and menaquinone biosynthesis.Biochem Biophys Res Commun. 1970 Sep 10;40(5):1090-5 PMID: 4926602
-
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
-
DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
-
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
-
Enzymes from Escherichia coli synthesize o-succinylbenzoic acid, an intermediate in menaquinone (vitamin K2) biosynthesis.J Biol Chem. 1981 Sep 25;256(18):9386-8 PMID: 7026552
-
Biosynthesis of o-succinylbenzoic acid in a men- Escherichia coli mutant requires decarboxylation of L-glutamate at the C-1 position.Biochemistry. 1981 Sep 1;20(18):5336-40 PMID: 6117313
-
Menaquinone (vitamin K2) biosynthesis: evidence that the Escherichia coli menD gene encodes both 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid synthase and alpha-ketoglutarate decarboxylase activities.J Bacteriol. 1992 Dec;174(24):8111-8 PMID: 1459959
-
Thiamine pyrophosphate requirement for o-succinylbenzoic acid synthesis in Escherichia coli and evidence for an intermediate.J Bacteriol. 1983 Feb;153(2):739-46 PMID: 6337125
-
Vitamin K (menaquinone) biosynthesis in bacteria: purification and probable structure of an intermediate prior to o-succinylbenzoate.Biochem Biophys Res Commun. 1985 Sep 16;131(2):956-60 PMID: 3902015
-
Vitamin K (menaquinone) biosynthesis in bacteria: high-performance liquid chromatographic assay of the overall synthesis of o-succinylbenzoic acid and of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid synthase.Anal Biochem. 1989 May 1;178(2):306-10 PMID: 2665567
-
Sequence and overexpression of the menD gene from Escherichia coli.J Bacteriol. 1989 Aug;171(8):4349-54 PMID: 2666397
-
The in vitro conversion of chorismate to isochorismate catalyzed by the Escherichia coli entC gene product. Evidence that EntA does not contribute to isochorismate synthase activity.J Biol Chem. 1989 Dec 5;264(34):20547-51 PMID: 2684979
-
Menaquinone (vitamin K2) biosynthesis: nucleotide sequence and expression of the menB gene from Escherichia coli.J Bacteriol. 1992 Aug;174(15):5057-62 PMID: 1629162
-
Biosynthesis of vitamin K (menaquinone) in bacteria.Microbiol Rev. 1982 Sep;46(3):241-80 PMID: 6127606
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
