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PMID: 833940 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Induction and biological properties of defective interfering particles of rabies virus.

Journal of virology ·Vol. 21 ·No. 2 ·1977-02-00 ·Pages 626-35

Wiktor TJ, Dietzschold B, Leamnson RN, Koprowski H

Abstract

A method for obtaining large quantities of defective interfering (DI) rabies virus particles that fulfill all the criteria delineated by Huang and Baltimore (1970) is described. The purified rabies DI virion was found to be much shorter (60 to 80 nm) than the complete virion (180 nm) and to have a viral genome of about half the size of normal rabies RNA but with all of the structural proteins of standard virions. Rabies DI virions were noninfectious for both cells in culture and for animals. As determined by in vitro and in vivo techniques, interference with the replication of standard virus was specific to rabies virus. The possible role of rabies DI virion in the pathogenicity of rabies virus infection and in the establishment of attenuated strains for use as live rabies vaccines is discussed.

MeSH Terms
Animals Cell Line Defective Viruses/growth & development,pathogenicity,ultrastructure Mice RNA, Viral/analysis Rabies virus/growth & development,pathogenicity,ultrastructure Viral Interference Viral Proteins/analysis Virus Replication
Chemicals
RNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wiktor T J
Dietzschold B
Leamnson R N
Koprowski H
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24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.21.2.626-635.1977
Published
1977-02-00
Pages
626-35
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353865
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.21.2.626-635.1977 PMC Full Text View journal details
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