Non-injection methods for the production of embryonic stem cell-embryo chimaeras.

Nature ·Vol. 365 ·No. 6441 ·1993-09-02 ·Pages 87-9

Wood SA, Allen ND, Rossant J, Auerbach A, Nagy A

Abstract

The simple aggregation of pluripotent ES cells with morulae-stage embryos can result in viable chimaeras at a similar frequency to that of blastocyst injection. The savings in time and equipment inherent in the aggregation techniques, however, make this approach well worth considering.

MeSH Terms

Animals Blastocyst/cytology Cell Aggregation Cells, Cultured Chimera Culture Techniques Embryo, Mammalian/cytology Embryology/methods Mice Mice, Inbred C57BL Microinjections Morula/cytology Stem Cells/cytology

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Wood S A

Department of Molecular Embryology, Max-Planck Institut für Immunbiologie, Freiburg, Germany.

Allen N D

Rossant J

Auerbach A

Nagy A

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/365087a0

Published

1993-09-02

Pages

87-9

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/365087a0 View journal details

Analysis Services

Non-injection methods for the production of embryonic stem cell-embryo chimaeras.

Contact

WeChat Official Account