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PMID: 8364601 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Positive-negative selection gene targeting with the diphtheria toxin A-chain gene in mouse embryonic stem cells.

Transgenic research ·Vol. 2 ·No. 4 ·1993-07-00 ·Pages 183-90

McCarrick JW, Parnes JR, Seong RH, Solter D, Knowles BB

Abstract

The diphtheria toxin A-chain gene was used in a positive-negative selection gene targeting vector to alter the CD4 gene which is transcriptionally silent in mouse embryonic stem cells. Expression of the toxin gene was driven by a constitutively active enhancer, yet the targeting construct exhibited only minimal transient toxicity while enriching for targeted clones 9- to 29-fold. Germline transmission of the stem cell-derived genome was obtained. These data suggest the usefulness of this diphtheria toxin A-chain cassette in replacement-type positive-negative selection vectors. Its potential for novel applications, particularly in the enrichment for 'hit-and-run' insertion-type vectors, is discussed.

Related Genes
CD4 DT-A HSV-TK PNS
MeSH Terms
Animals CD4 Antigens/genetics Cell Line DNA Diphtheria Toxin/genetics Embryo, Mammalian/cytology Genetic Vectors Mice Mutagenesis Peptide Fragments/genetics Stem Cells
Chemicals
CD4 Antigens Diphtheria Toxin Peptide Fragments diphtheria toxin fragment A DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
McCarrick J W
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Parnes J R
Seong R H
Solter D
Knowles B B
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Article Info
Journal
Transgenic research
Abbr.
Transgenic Res
ISSN
0962-8819
DOI
10.1007/BF01977348
Published
1993-07-00
Pages
183-90
Language
English
Region
Netherlands
NLM ID
9209120
Subset
IM
Grants
NCI NIH HHS · CA 10815 · United States
NCI NIH HHS · CA 18470 · United States
NCI NIH HHS · CA 21124 · United States
External Links
PubMed source DOI: 10.1007/BF01977348 View journal details
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