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PMID: 8367492 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Ectopic neural expression of a floor plate marker in frog embryos injected with the midline transcription factor Pintallavis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 17 ·1993-09-01 ·Pages 8268-72

Ruiz i Altaba A, Cox C, Jessell TM, Klar A

Abstract

The floor plate, a cell group that develops at the midline of the neural plate in response to inductive signals from the notochord, has been implicated in the control of dorsoventral neural pattern. The frog Pintallavis gene, encoding a member of the HNF-3/fork head transcription factor family, is expressed in the notochord and in midline neural plate cells that give rise to the floor plate. To examine whether Pintallavis might be involved in regulating the differentiation of the floor plate, we ectopically expressed Pintallavis by injection of synthetic mRNA into two-cell frog embryos. Injection of Pintallavis mRNA resulted in the ectopic expression of F-spondin, a gene encoding a floor plate-specific adhesion molecule, at the dorsal midline of the neural tube. The expression of Pintallavis in midline cells may therefore contribute to the establishment of the floor plate fate.

MeSH Terms

Albinism Amino Acid Sequence Animals Cell Adhesion Molecules/genetics Embryo, Nonmammalian/physiology Forkhead Transcription Factors Gene Library Growth Substances Intercellular Signaling Peptides and Proteins Membrane Glycoproteins/genetics Molecular Sequence Data Multigene Family Nerve Tissue Proteins/biosynthesis,genetics Neurons/physiology Peptides RNA, Messenger/metabolism Sequence Homology, Amino Acid Thrombospondins Transcription Factors/biosynthesis,genetics,metabolism Xenopus Proteins Xenopus laevis/embryology

Chemicals

Cell Adhesion Molecules Forkhead Transcription Factors FoxA4 protein, Xenopus Growth Substances Intercellular Signaling Peptides and Proteins Membrane Glycoproteins Nerve Tissue Proteins Peptides RNA, Messenger Thrombospondins Transcription Factors Xenopus Proteins spon1 protein, Xenopus

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ruiz i Altaba A

Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.

Cox C

Jessell T M

Klar A

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.90.17.8268

Published

1993-09-01

Pages

8268-72

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC47330

Subset

Databases

GENBANK

L09123

External Links

PubMed source DOI: 10.1073/pnas.90.17.8268 PMC Full Text View journal details

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Ectopic neural expression of a floor plate marker in frog embryos injected with the midline transcription factor Pintallavis.

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