Home LiteratureArticle Details
PMID: 8373591 Published · ppublish English Research Support, Non-U.S. Gov't Technical Report

A rapid optimized protocol for downward alkaline Southern blotting of DNA.

BioTechniques ·Vol. 15 ·No. 2 ·1993-08-00 ·Pages 260-2

Koetsier PA, Schorr J, Doerfler W

Abstract

A simple and efficient 2.5-h Southern blotting procedure is described that uses 0.4 M NaOH to transfer DNA in a downward direction. The resulting blots give signals that are both sharper and 30% stronger than those obtained by conventional upward-transferred blots.

MeSH Terms
Animals Blotting, Southern/methods DNA/analysis Mice Sodium Hydroxide
Chemicals
Sodium Hydroxide DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Koetsier P A
Institute for Genetics, University of Cologne, FRG.
Schorr J
Doerfler W
Article Info
Journal
BioTechniques
Abbr.
Biotechniques
ISSN
0736-6205
Published
1993-08-00
Pages
260-2
Language
English
Region
England
NLM ID
8306785
Subset
IM
External Links
PubMed source View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2