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PMID: 8376945 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interleukin 2 activates extracellular signal-regulated protein kinase 2.

The Journal of experimental medicine ·Vol. 178 ·No. 4 ·1993-10-01 ·Pages 1429-34

Perkins GR, Marvel J, Collins MK

Abstract

Interleukin 2 (IL-2) stimulated activation of the 42-kD extracellular signal-regulated kinase 2 (Erk2) in murine IL-3-dependent cells, expressing either high or intermediate affinity IL-2 receptors. Activation was both rapid, occurring within 5 min of IL-2 addition, and prolonged, remaining elevated for 30 min. Activation of Erk2 appeared to be necessary for IL-2 stimulation of proliferation, as deletion of a region of the cytoplasmic domain of the IL-2 receptor beta chain, essential for IL-2 stimulation of proliferation, abolished Erk2 activation by IL-2. Furthermore, cells that had been deprived of cytokine for 24 h were then refractory to IL-2 stimulation of both Erk2 activity and proliferation. However, elevation of Erk2 activity was not sufficient to stimulate proliferation, as protein kinase C activation stimulated Erk2 activity but not DNA synthesis. Also, cells exposed to IL-2 in the presence of rapamycin showed full Erk2 activation but not DNA synthesis. These data suggest that IL-2 must stimulate both Erk2 activity and a further pathway(s) to trigger cell proliferation.

MeSH Terms
Animals Cell Division Cell Line Enzyme Activation Humans Interleukin-2/pharmacology Mice Mitogen-Activated Protein Kinase 1 Protein Serine-Threonine Kinases/metabolism Receptors, Interleukin-2/metabolism Signal Transduction
Chemicals
Interleukin-2 Receptors, Interleukin-2 Protein Serine-Threonine Kinases Mitogen-Activated Protein Kinase 1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Perkins G R
Chester Beatty Laboratories, Institute of Cancer Research, London, England.
Marvel J
Collins M K
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.178.4.1429
Published
1993-10-01
Pages
1429-34
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191204
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.178.4.1429 PMC Full Text View journal details
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