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PMID: 8381785 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

180-kD ribosome receptor is essential for both ribosome binding and protein translocation.

The Journal of cell biology ·Vol. 120 ·No. 4 ·1993-02-00 ·Pages 853-63

Savitz AJ, Meyer DI

Abstract

We have previously isolated a 180-kD ribosome receptor (p180) from mammalian rough ER that, when incorporated into liposomes, bound ribosomes with an affinity similar to intact membranes. To directly assess the contribution of p180 to ribosome binding as well as protein translocation, monoclonal antibodies were used to selectively deplete p180 from the detergent extracts of rough ER membranes used in the preparation of translocation-competent proteoliposomes. Proteoliposomes prepared from p180-depleted extracts showed a reduction in ribosome binding to the level of trypsin-inactivated controls as well as a loss in their ability to cotranslationally translocate two different secretory protein precursors. When purified p180 was added back to depleted extracts before proteoliposome formation, both ribosome binding and translocation activity were restored. In addition, the monoclonal antibodies, as well as their Fab' fragments, were able to inhibit ribosome binding and protein translocation when bound to intact rough microsomes. These data provide direct evidence that the 180-kD ribosome receptor is essential for ribosome binding and for the translocation of nascent proteins across the membrane of the rough ER.

MeSH Terms
Animals Biological Transport Dogs Endoplasmic Reticulum/metabolism In Vitro Techniques Membrane Proteins/metabolism Molecular Weight Proteins/metabolism Proteolipids Receptors, Cell Surface/immunology,metabolism Receptors, Cytoplasmic and Nuclear Ribosomes/metabolism
Chemicals
Membrane Proteins Proteins Proteolipids Receptors, Cell Surface Receptors, Cytoplasmic and Nuclear proteoliposomes ribosome receptor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Savitz A J
Department of Biological Chemistry, University of California, Los Angeles School of Medicine.
Meyer D I
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36 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.120.4.853
Published
1993-02-00
Pages
853-63
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2200074
Subset
IM
Grants
NIGMS NIH HHS · GM 38538 · United States
NIGMS NIH HHS · GM0-8042-09 · United States
External Links
PubMed source DOI: 10.1083/jcb.120.4.853 PMC Full Text View journal details
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