An enzymatic method for the histochemical localization of free and esterified cholesterol separately.

The Histochemical journal ·Vol. 9 ·No. 2 ·1977-03-00 ·Pages 197-204

Emeis JJ, Van Gent CM, Van Sabben CM

Abstract

An enzymatic method for the histochemical localization of cholesterol is presented. It makes possible the localization of free cholesterol, cholesterol esters, or both and is compatible with routine histological staining procedures. The method is based on the production of H2O2 from free cholesterol by cholesterol oxidase. Sites of peroxide production are visualized by a brown reaction product formed in a peroxidase-catalysed reaction between diaminobenzidine and H2O2. Cholesterol esters can be demonstrated as cholesterol after hydrolysis by cholesterol ester hydrolase. Some examples of the application of the method are given.

MeSH Terms

Adrenal Glands/analysis Animals Aorta/analysis Cholesterol/analysis Cholesterol Esters/analysis Histocytochemistry/methods Humans Hydroxysteroid Dehydrogenases Kidney/analysis Liver/analysis Lung/analysis Peroxidases Rabbits Rats Spleen/analysis Sterol Esterase

Chemicals

Cholesterol Esters Cholesterol Hydroxysteroid Dehydrogenases Peroxidases Sterol Esterase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Emeis J J

Van Gent C M

Van Sabben C M

References (6)

6 references, click to expand

A perchloric acid-naphthoquinone method for the histochemical localization of cholesterol.
Nature. 1961 Oct 28;192:331-2 PMID: 13859298
Cholesterol oxidase. Further studies of substrate specificity in relation to the analytical characterisation of steroids.
J Chromatogr. 1975 Oct 29;112:499-511 PMID: 1184684
A method for the histochemical differentiation of cholesterol and its esters.
J Biophys Biochem Cytol. 1956 Mar 25;2(2):213-4 PMID: 13319382
Studies in lipid histochemistry. VI. Problems of extraction with acetone in lipid histochemistry.
Histochemie. 1971;28(1):68-87 PMID: 5137659
Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum.
Clin Chem. 1973 Dec;19(12):1350-6 PMID: 4757363
Enzymatic determination of total serum cholesterol.
Clin Chem. 1974 Apr;20(4):470-5 PMID: 4818200

Article Info

Journal

The Histochemical journal

Abbr.

Histochem J

ISSN

0018-2214

DOI

10.1007/BF01003631

Published

1977-03-00

Pages

197-204

Language

English

Region

Netherlands

NLM ID

0163161

Subset

External Links

PubMed source DOI: 10.1007/BF01003631

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An enzymatic method for the histochemical localization of free and esterified cholesterol separately.

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