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PMID: 8392878 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast.

Molecular biology of the cell ·Vol. 4 ·No. 5 ·1993-05-00 ·Pages 511-21

Rohrer J, Bénédetti H, Zanolari B, Riezman H

Abstract

The Saccharomyces cerevisiae alpha-pheromone receptor, a polytopic, G protein-coupled, membrane protein, is internalized after binding of alpha-factor. Mutational analysis suggested that the first 39 residues of the receptor's cytoplasmic tail carries sufficient information for internalization. A point mutation in one of these 39 residues, K337 to R337, renders the receptor nonfunctional for endocytosis. Other residues, D335 and S338, contribute to the efficiency of internalization. When the sequence DAKSS is added onto a severely truncated receptor, endocytosis of the receptor is restored, showing that this sequence functions to mediate or to signal interaction with the endocytic machinery. Analysis of pheromone response and recovery in strains expressing mutant receptors suggests that receptor internalization is not important for response but contributes to recovery from pheromone.

MeSH Terms
Amino Acid Sequence Binding Sites Endocytosis GTP-Binding Proteins/metabolism Genotype Mating Factor Molecular Sequence Data Peptides/metabolism,pharmacology Pheromones/metabolism,pharmacology Point Mutation Receptors, Cell Surface/chemistry,metabolism Receptors, Mating Factor Receptors, Peptide Saccharomyces cerevisiae/genetics,metabolism Signal Transduction Transcription Factors
Chemicals
Peptides Pheromones Receptors, Cell Surface Receptors, Mating Factor Receptors, Peptide Transcription Factors Mating Factor GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rohrer J
Biocenter of the University of Basel, Switzerland.
Bénédetti H
Zanolari B
Riezman H
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.4.5.511
Published
1993-05-00
Pages
511-21
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC300954
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.4.5.511 PMC Full Text View journal details
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