The signal transduction pathway for VGF expression due to NGF is different from that due to bFGF in PC12h cell.

Using a polyclonal antibody against VGF, we examined the effects of various agents for its expression in PC12h cells. The results obtained were follows; 1) Basic fibroblast growth factor (bFGF), forskolin, dibutyryl cyclic AMP (dbcAMP), and nerve growth factor (NGF) induced the VGF protein. 2) The induction of VGF protein by NGF was inhibited by methylthioadenosine (MTA), but its induction by bFGF was not. 3) The induction of VGF protein by NGF was blocked by staurosporine, but its induction by bFGF was not. These findings suggest that the induction of VGF by NGF is distinct from that by bFGF in terms of sensitivities to MTA and staurosporine.