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PMID: 8396686 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional and genetic plasticities of the poliovirus genome: quasi-infectious RNAs modified in the 5'-untranslated region yield a variety of pseudorevertants.

Journal of virology ·Vol. 67 ·No. 10 ·1993-10-00 ·Pages 6309-16

Gmyl AP, Pilipenko EV, Maslova SV, Belov GA, Agol VI

Abstract

Poliovirus RNA species with nucleotides 564 to 571 deleted or with a secondary structure domain (positions 564 to 629) replaced by a shorter irregular oligonucleotide have been engineered previously; these RNAs have been considered quasi-infectious (yielding a single late revertant plaque) and dead, respectively (E. Pilipenko, A. Gmyl, Y. Svitkin, S. Maslova, A. Sinyakov, and V. Agol, Cell 68:119-131, 1992). By using large amounts of these RNAs for transfections, revertant clones with a great variety of genetic changes (point mutations, insertions of foreign sequences, short or extended deletions) were isolated. The pattern of these changes supported the notion that an appropriately spaced oligopyrimidine-AUG tandem is important for efficient poliovirus RNA translation. Structural features within and around this tandem modulated the initiation efficiency. The functional and genetic plasticities of the poliovirus genome are briefly discussed.

MeSH Terms
Animals Base Sequence Biological Evolution Cells, Cultured Cloning, Molecular Genome, Viral Genotype Molecular Sequence Data Mutagenesis, Insertional Mutagenesis, Site-Directed Nucleic Acid Conformation Oligodeoxyribonucleotides Point Mutation Poliovirus/genetics,pathogenicity,physiology Polymerase Chain Reaction RNA, Viral/chemistry,genetics Restriction Mapping Sequence Deletion Templates, Genetic Viral Plaque Assay Virulence/genetics Virus Replication
Chemicals
Oligodeoxyribonucleotides RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gmyl A P
Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow Region.
Pilipenko E V
Maslova S V
Belov G A
Agol V I
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.10.6309-6316.1993
Published
1993-10-00
Pages
6309-16
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC238059
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.67.10.6309-6316.1993 PMC Full Text View journal details
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