[New site site-specific endonuclease and methylase from Bacillus licheniformis 736].

Novye saĭt-spetsificheskie éndonukleaza i metilaza iz Bacillus licheniformis 736.

Biokhimiia (Moscow, Russia) ·Vol. 58 ·No. 8 ·1993-08-00 ·Pages 1139-53

Matvienko NN, Kramarov VM, Zheleznaia LA, Matvienko NI

Abstract

The site-specific endonuclease R Bli736I and methylase M Bli736I have been isolated from the Bacillus licheniformis strain 736 by blue-agarose, hydroxyapatite-Ultragel and heparin-Sepharose chromatography. The enzymes are free from interfering impurities. R Bli736I recognizes the 5'-GGTCTCN-3' decreases and decreases 5'-NNNNNGAGACC-3' sequences on the DNA and cleaves the DNA as indicated by arrows to form single-stranded 4-nucleotide 5'-protruding termini. This enzyme is an isoschizomer of Eco3II isolated from E. coli.

MeSH Terms

Bacillus/enzymology Base Sequence Chromatography, Gel DNA, Viral/metabolism DNA-Cytosine Methylases/isolation & purification,metabolism Deoxyribonucleases, Type II Site-Specific/isolation & purification,metabolism Electrophoresis, Agar Gel Molecular Sequence Data Site-Specific DNA-Methyltransferase (Adenine-Specific)/isolation & purification,metabolism Substrate Specificity

Chemicals

DNA, Viral DNA modification methylase Eco31I DNA-Cytosine Methylases Site-Specific DNA-Methyltransferase (Adenine-Specific) endodeoxyribonuclease Eco31I Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Matvienko N N

Kramarov V M

Zheleznaia L A

Matvienko N I

Article Info

Journal

Biokhimiia (Moscow, Russia)

Abbr.

Biokhimiia

ISSN

0320-9725

Published

1993-08-00

Pages

1139-53

Language

rus

Region

Russia (Federation)

NLM ID

0372667

Subset

External Links

PubMed source

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[New site site-specific endonuclease and methylase from Bacillus licheniformis 736].

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