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PMID: 8400121 Published · ppublish English Journal Article

Nuclease sensitivity and functional analysis of a maize histone H3 gene promoter.

Plant molecular biology ·Vol. 22 ·No. 6 ·1993-09-00 ·Pages 1007-15

Brignon P, Lepetit M, Gigot C, Chaubet N

Abstract

A 1 kb region of a maize H3 histone gene promoter has been analysed at a structural and functional level. Micrococcal nuclease digestion of isolated nuclei showed that the promoter region is organized into nucleosomes but a zone extending over approximately one nucleosome (20 to 230 bp upstream of the TATA box) displays remarkable accessibility to digestion. Three DNase I-hypersensitive sites were found within this zone at the vicinity of consensus sequences, some of which are already known to act as cis elements. This promoter region is able to direct faithful expression of the GUS reporter gene in meristematic tissues of transgenic tobacco plants.

MeSH Terms
Base Sequence Chromatin/metabolism,ultrastructure DNA Deoxyribonuclease I/metabolism Glucuronidase/biosynthesis,genetics Histones/genetics Micrococcal Nuclease/metabolism Molecular Sequence Data Nucleosomes/ultrastructure Plants, Genetically Modified Plants, Toxic Promoter Regions, Genetic Tobacco/genetics Zea mays/genetics
Chemicals
Chromatin Histones Nucleosomes DNA Deoxyribonuclease I Micrococcal Nuclease Glucuronidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brignon P
Institut de Biologie Moléculaire des Plantes du C.N.R.S., Université Louis Pasteur, Strasbourg, France.
Lepetit M
Gigot C
Chaubet N
References (17)
17 references, click to expand
  1. Highly conserved hexamer, octamer and nonamer motifs are positive cis-regulatory elements of the wheat histone H3 gene.
    FEBS Lett. 1992 Mar 30;300(2):167-70 PMID: 1563517
  2. A plant histone gene promoter can direct both replication-dependent and -independent gene expression in transgenic plants.
    Mol Gen Genet. 1992 Jan;231(2):276-85 PMID: 1736097
  3. DNase I hypersensitivity and expression of the Shrunken-1 gene of maize.
    Plant Mol Biol. 1987 May;8(3):251-64 PMID: 24301129
  4. An internal standard improves the reliability of transient expression studies in plant protoplasts.
    Plant Cell Rep. 1991 Oct;10(8):401-5 PMID: 24221733
  5. A 126 bp fragment of a plant histone gene promoter confers preferential expression in meristems of transgenic Arabidopsis.
    Plant J. 1992 May;2(3):291-300 PMID: 1303796
  6. Tissue-specific expression of a wheat high molecular weight glutenin gene in transgenic tobacco.
    Plant Cell. 1989 Jun;1(6):569-78 PMID: 2535510
  7. The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
    Nature. 1980 Aug 28;286(5776):854-60 PMID: 6774262
  8. DNase I hypersensitive sites in the 5'-region of the maize Shrunken gene in nuclei from different organs.
    Mol Gen Genet. 1988 May;212(2):351-9 PMID: 2841573
  9. Multilevel regulation of replication-dependent histone genes.
    Trends Genet. 1988 Jul;4(7):187-91 PMID: 3070866
  10. Cell-specific expression of plant histone H2A genes.
    Plant Cell. 1991 Jul;3(7):657-65 PMID: 1841722
  11. Nucleosome positioning: occurrence, mechanisms, and functional consequences.
    Prog Nucleic Acid Res Mol Biol. 1991;40:143-84 PMID: 2031082
  12. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
    EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
  13. DNA-binding protein(s) interacts with a conserved nonameric sequence in the upstream regions of wheat histone genes.
    FEBS Lett. 1988 Nov 7;239(2):319-23 PMID: 3181434
  14. Regulation of the maize rab17 gene promoter in transgenic heterologous systems.
    Plant Mol Biol. 1991 Nov;17(5):985-93 PMID: 1932688
  15. Selected topics in chromatin structure.
    Annu Rev Genet. 1985;19:485-536 PMID: 3909948
  16. Multiple functions of nucleosomes and regulatory factors in transcription.
    Trends Biochem Sci. 1993 Mar;18(3):90-5 PMID: 8480368
  17. Organ-specific expression of different histone H3 and H4 gene subfamilies in developing and adult maize.
    Plant Mol Biol. 1991 Oct;17(4):935-40 PMID: 1912509
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00028973
Published
1993-09-00
Pages
1007-15
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00028973 View journal details
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