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PMID: 8407799 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Lactose permease mutants which transport (malto)-oligosaccharides.

Journal of bacteriology ·Vol. 175 ·No. 19 ·1993-10-00 ·Pages 6269-75

Olsen SG, Greene KM, Brooker RJ

Abstract

Lactose permease mutants, which were previously isolated in sugar specificity studies, were screened for their abilities to transport the trisaccharide maltotriose. Six multiple mutants (e.g., five double mutants and one triple mutant) were identified as forming fermentation-positive colonies on maltotriose MacConkey plates and were also shown to grow on maltotriose minimal plates. All of these multiple mutants contained a combination of two or three amino acid substitutions at position 177, 236, 306, or 322 within the permease. In contrast, none of the corresponding single mutants at these locations were observed to exhibit an enhanced rate of maltotriose transport. In whole-cell assays, the multiple mutants were shown to transport relatively long alpha-nitrophenylglucoside (alpha NPG) molecules. In certain cases, alpha NPG molecules containing up to four glucose residues in addition to the nitrophenyl group were shown to be transported to a significant degree. Overall, the abilities of lactose permease mutants to transport maltotriose and long alpha NPGs are discussed with regard to the dimensions of the sugar and the mechanism of sugar transport.

MeSH Terms
Biological Transport Carbohydrate Sequence Escherichia coli/enzymology,genetics Escherichia coli Proteins Fermentation Kinetics Membrane Transport Proteins/genetics,metabolism Molecular Sequence Data Monosaccharide Transport Proteins Oligosaccharides/metabolism Plasmids Point Mutation Substrate Specificity Symporters
Chemicals
Escherichia coli Proteins LacY protein, E coli Membrane Transport Proteins Monosaccharide Transport Proteins Oligosaccharides Symporters maltooligosaccharides lactose permease
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Olsen S G
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108.
Greene K M
Brooker R J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.19.6269-6275.1993
Published
1993-10-00
Pages
6269-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206723
Subset
IM
Grants
NIAID NIH HHS · AI24204 · United States
External Links
PubMed source DOI: 10.1128/jb.175.19.6269-6275.1993 PMC Full Text View journal details
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