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PMID: 8413604 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Crystal structure of a yeast TBP/TATA-box complex.

Nature ·Vol. 365 ·No. 6446 ·1993-10-07 ·Pages 512-20

Kim Y, Geiger JH, Hahn S, Sigler PB

Abstract

The 2.5 A crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primarily hydrophobic interface with the entire under-surface of the TBP saddle. The severe bend and a positive writhe radically alter the trajectory of the flanking B-form DNA.

MeSH Terms
Amino Acid Sequence Base Sequence Computer Graphics Crystallography, X-Ray Cytochrome c Group/genetics Cytochromes c DNA/chemistry Hydrogen Bonding Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic Protein Binding Protein Conformation Recombinant Proteins/chemistry Saccharomyces cerevisiae/chemistry Saccharomyces cerevisiae Proteins Structure-Activity Relationship TATA Box Transcription Factor TFIIA Transcription Factor TFIID Transcription Factors/chemistry,metabolism
Chemicals
CYC1 protein, S cerevisiae Cytochrome c Group Recombinant Proteins Saccharomyces cerevisiae Proteins Transcription Factor TFIIA Transcription Factor TFIID Transcription Factors Cytochromes c DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kim Y
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510.
Geiger J H
Hahn S
Sigler P B
Article Info
Journal
Nature
Abbr.
Nature
ISSN
0028-0836
DOI
10.1038/365512a0
Published
1993-10-07
Pages
512-20
Language
English
Region
England
NLM ID
0410462
Subset
IM
Corrections
CommentIn
PMID: 8413598
External Links
PubMed source DOI: 10.1038/365512a0 View journal details
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