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PMID: 8421051 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Ran/TC4: a small nuclear GTP-binding protein that regulates DNA synthesis.

The Journal of cell biology ·Vol. 120 ·No. 2 ·1993-01-00 ·Pages 313-23

Ren M, Drivas G, D'Eustachio P, Rush MG

Abstract

Ran/TC4, first identified as a well-conserved gene distantly related to H-RAS, encodes a protein which has recently been shown in yeast and mammalian systems to interact with RCC1, a protein whose function is required for the normal coupling of the completion of DNA synthesis and the initiation of mitosis. Here, we present data indicating that the nuclear localization of Ran/TC4 requires the presence of RCC1. Transient expression of a Ran/TC4 protein with mutations expected to perturb GTP hydrolysis disrupts host cell DNA synthesis. These results suggest that Ran/TC4 and RCC1 are components of a GTPase switch that monitors the progress of DNA synthesis and couples the completion of DNA synthesis to the onset of mitosis.

Related Genes
H-RAS Ran TC4
MeSH Terms
Amino Acid Sequence Animals Cell Line Cell Nucleus/metabolism,ultrastructure Chromatin/physiology,ultrastructure Chromosomes/physiology,ultrastructure Cricetinae DNA/biosynthesis DNA Replication Fluorescent Antibody Technique GTP Phosphohydrolases/metabolism GTP-Binding Proteins/genetics,metabolism Humans Kidney Mice Mitosis Molecular Sequence Data Mutagenesis, Site-Directed Nuclear Proteins/genetics,metabolism Oligodeoxyribonucleotides Sequence Homology, Amino Acid Subcellular Fractions/metabolism Transfection ran GTP-Binding Protein
Chemicals
Chromatin Nuclear Proteins Oligodeoxyribonucleotides DNA GTP Phosphohydrolases GTP-Binding Proteins ran GTP-Binding Protein
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ren M
Department of Cell Biology, New York University Medical Center, NY 10016.
Drivas G
D'Eustachio P
Rush M G
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32 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.120.2.313
Published
1993-01-00
Pages
313-23
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119524
Subset
IM
Grants
NIGMS NIH HHS · GM07827 · United States
NCRR NIH HHS · RR083990 · United States
Databases
GENBANK
M31469
External Links
PubMed source DOI: 10.1083/jcb.120.2.313 PMC Full Text View journal details
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